RNase A
RNase A (Ribonuclease A) is a small, stable endonuclease derived from bovine pancreas that catalyzes the hydrolysis of single-stranded RNA by cleaving at the 3' end of pyrimidine residues. This reaction occurs without the need for metal ions or cofactors, making RNase A a highly efficient model for studying enzyme stability, protein folding, and catalytic mechanisms. Due to its specificity for pyrimidines and robust structure, RNase A is widely used in biomedicine and biochemistry research, as well as for understanding RNA processing and degradation.
Species of Origin:
Bovine pancreas
Activity:
>80 U/mg
Form:
Lyophilized
Unit Definition:
One unit of RNase A is defined as the amount of enzyme that can degrade RNA at 25°C, resulting in an increase of 1.0 absorbance unit at 260 nm.
Stability & Storage:
This product is stable after storage at:
● -20°C for 12 months in lyophilized state from date of receipt.
● -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Reconstitution:
It is recommended to reconstitute the lyophilized protein in DEPC-treated water to a concentration range of 10–100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping Conditions:
Blue ice
Bovine pancreas
Activity:
>80 U/mg
Form:
Lyophilized
Unit Definition:
One unit of RNase A is defined as the amount of enzyme that can degrade RNA at 25°C, resulting in an increase of 1.0 absorbance unit at 260 nm.
Stability & Storage:
This product is stable after storage at:
● -20°C for 12 months in lyophilized state from date of receipt.
● -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Reconstitution:
It is recommended to reconstitute the lyophilized protein in DEPC-treated water to a concentration range of 10–100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping Conditions:
Blue ice
